2 removing me and then them, for you once (4)
I believe the answer is:
thee
'you once' is the definition.
(historical second person pronoun)
I don't understand how the rest of the clue works.
Can you help me to learn more?
(Other definitions for thee that I've seen before include "Archaic form of 'you'" , "you many years ago" , "Poetically you" , "Old objective second person singular" , "Quaker 'you'" .)