A group of six tsetse flies (6)
I believe the answer is:
sestet
'a group of six' is the definition.
(I've seen this before)
'tsetse flies' is the wordplay.
'flies' is an anagram indicator.
'tsetse' is an anagram of 'SESTET'.
(Other definitions for sestet that I've seen before include "Group of six" , "Tsetse (anag.)" , "Six together" , "Set of six performers" , "musical ensemble" .)