A lady looking up and down (5)
I believe the answer is:
madam
'up and down' is the definition.
I don't understand how the rest of the clue works.
(Other definitions for madam that I've seen before include "Formal address to a woman - precocious little girl" , "'Call Me ---' (I. Berlin)" , "Term of address to a woman" , "Polite form of address for a woman" , "precocious schoolgirl?" .)