A New Testament colonist (3)
I believe the answer is:
ant
'colonist' is the definition.
(ants live in colonies)
'a new testament' is the wordplay.
'new testament' becomes 'nt'.
'a'+'nt'='ANT'
(Other definitions for ant that I've seen before include "one living in colonies" , "Worker on the hill" , "Small busy insect" , "Colonial insect?" , "that may be a soldier" .)