A role in isolation? (5)
I believe the answer is:
apart
'in isolation?' is the definition.
(I've seen this before)
'a role' is the wordplay.
'role' becomes 'part' (eg a part or role in a film).
'a'+'part'='APART'
(Other definitions for apart that I've seen before include "Unconnected" , "Independently" , "set aside" , "First Christmas present from my love" , "In pieces; separate" .)