A tune from 'The Fairie Queen (3)
I believe the answer is:
air
'a tune' is the definition.
(air can mean a piece of music)
'from 'the fairie queen' is the wordplay.
'from' indicates the answer is hidden within the clue.
'AIR' is hidden within 'fairie queen'.
(Other definitions for air that I've seen before include "Divulge" , "See 21" , "Simple composition" , "Element; tune" , "Tune; mien" .)