Ablest men upset with this business? (13)
I believe the answer is:
establishment
'business?' is the definition.
(thesaurus)
'ablest men upset with this' is the wordplay.
'upset with' is an anagram indicator.
'ablest'+'men'='ablestmen'
'ablestmen' with its letters anagrammed with 'this' makes 'ESTABLISHMENT'.
(Other definitions for establishment that I've seen before include "system" , "powers that be" , "Power-holding class" , "being incorporated in this" , "Governing body" .)