Ace getting cheeky again? (6)
I believe the answer is:
afresh
'again?' is the definition.
(synonyms)
'ace getting cheeky' is the wordplay.
'ace' becomes 'a' (abbreviation).
'getting' means one lot of letters go next to another.
'cheeky' becomes 'fresh' (I've seen this before).
'a'+'fresh'='AFRESH'
(Other definitions for afresh that I've seen before include "Academy award" , "In a different way" , "Anew, from the beginning" , "In a new way" , "Newly" .)