Act last from Art Pepper (3)
I believe the answer is:
dot
'pepper' is the definition.
(I've seen this in another clue)
'act last from art' is the wordplay.
'act' becomes 'do' (to do is to act or execute a task).
'last from' indicates one should take the final letters.
The final letter of 'art' is 't'.
'do'+'t'='DOT'
(Other definitions for dot that I've seen before include "Spot, speck" , "Morse symbol; spot" , "Point; speck" , "Internet punctuation mark" , "Flyspeck: period in a web address" .)