After 1 in the morning, I'm not sure I have targets (5)

I believe the answer is:

aimer

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'i have targets' is the definition.
I don't know anything about this answer so I cannot judge whether this works.

'after 1 in the morning i'm not sure' is the wordplay.
'after' is a charade indicator (letters next to each other).
'1' becomes 'I' (roman numeral).
'in the' is an insertion indicator.
'morning' becomes 'am'.
'i'm not sure' becomes 'er' (expression of hesitation).
'i' going within 'am' is 'aim'.
'aim'+'er' is 'AIMER'.

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