American Symphony Orchestra in Newark, last of all (4)
I believe the answer is:
yank
'american' is the definition.
(Yank can informally mean an American)
'symphony orchestra in newark last of all' is the wordplay.
'last of all' indicates one should take the final letters.
The final letters of 'symphony orchestra in newark' are 'YANK'.
(Other definitions for yank that I've seen before include "Jerk, pull suddenly" , "Sharp movement" , "American - jerk" , "Wrench" , "Pull hard suddenly" .)