Bar last pair, voicing pique all at once (5)
I believe the answer is:
tutti
'all at once' is the definition.
(musical instruction)
I cannot understand how the remainder of the clue works.
(Other definitions for tutti that I've seen before include "(Musically) all play together" , "Every instrument to play" , "[MUSICIAN] play together" , "All (musical players)" , "Piece together" .)