Boffin no longer frisky? (6)
I believe the answer is:
expert
'boffin' is the definition.
'no longer frisky?' is the wordplay.
'no longer' becomes 'ex'.
'frisky?' becomes 'pert' (both can mean lively).
'ex'+'pert'='EXPERT'
(Other definitions for expert that I've seen before include "Specialist, pundit" , "Skilled workman or specialist" , "One has specialist knowledge or ability" , "Clever" , "Masterly" .)