Bossy young girl running up and down (5)
I believe the answer is:
madam
I believe this clue is a double definition.
'bossy young girl' is the first definition.
'running up and down' is the second definition.
(Other definitions for madam that I've seen before include "formidable woman" , "How one may address a woman" , "Courteous form of address to a woman" , "Bossy girl" , "Palindrome used in speaking politely to a woman" .)