Buffoonery of liar had Queen troubled (12)
I believe the answer is:
harlequinade
'buffoonery' is the definition.
('harlequinade' can be a synonym of 'buffoonery')
'liar had queen troubled' is the wordplay.
'troubled' indicates anagramming the letters.
'liar'+'had'+'queen'='liarhadqueen'
'liarhadqueen' is an anagram of 'HARLEQUINADE'.
'of' acts as a link.
(Other definitions for harlequinade that I've seen before include "Pantomime; buffoonery" , "Part of pantomime" , "Traditional pantomime episode" .)