Charlie worried about queen’s old car (5)
I believe the answer is:
crate
'old car' is the definition.
(informal term for an old car)
'charlie worried about queen's' is the wordplay.
'charlie' becomes 'c' (phonetic alphabet: alpha, bravo, charlie etc.).
'worried' becomes 'ate' (something worrying may eat someone up).
'about' is a charade indicator (letters next to each other).
'queen' becomes 'r' (abbreviation for regina, e.g. in ER ).
'ate' put after 'r' is 'rate'.
'c'+'rate'='CRATE'
(Other definitions for crate that I've seen before include "Packing container" , "Jalopy" , "Wooden box" , "old banger" , "Old car" .)