Check one with Queen getting more irritable (7)
I believe the answer is:
testier
'more irritable' is the definition.
('testy' can be a synonym of 'irritable')
'check one with queen' is the wordplay.
'check' becomes 'test' ('test' can be a synonym of 'check'**).
'one' becomes 'i' (Roman numeral).
'with' says to put letters next to each other.
'queen' becomes 'er' (abbreviation for Elizabeth Regina).
'test'+'i'+'er'='TESTIER'
'getting' acts as a link.
(Other definitions for testier that I've seen before include "more crusty" , "More likely to become annoyed" , "more irritable" .)