Chuck holds animal, looking displeased (8)
I believe the answer is:
scowling
'looking displeased' is the definition.
(I've seen this before)
'chuck holds animal' is the wordplay.
'chuck' becomes 'sling' (chuck means throw as does sling).
'holds' is an insertion indicator.
'animal' becomes 'cow' (cow is a kind of animal).
'sling' placed around 'cow' is 'SCOWLING'.
(Other definitions for scowling that I've seen before include "glowering" , "looking unhappy?" , "Frowning with displeasure" , "looking displeased" , "Lowering" .)