Dark time near end of twilight (5)
I believe the answer is:
night
'dark time' is the definition.
(I've seen this before)
'near end of twilight' is the wordplay.
'near' becomes 'nigh' (synonyms).
'end of' indicates one should take the final letters.
The final letter of 'twilight' is 't'.
'nigh'+'t'='NIGHT'
(Other definitions for night that I've seen before include "For most, the time for sleep" , "'She walks in beauty, like the . . . . .' (Byron)" , "Time for bed" , "8d [DARKNESS]" , "period of gloom" .)