Demand from assassins is threat (6)
I believe the answer is:
insist
'demand' is the definition.
(thesaurus)
'from assassins is threat' is the wordplay.
'from' indicates a hidden word.
'INSIST' can be found hidden inside 'assassins is threat'.
(Other definitions for insist that I've seen before include "'Demand, make sure (6)'" , "Assert firmly" , "Declare or demand emphatically" , "Press" , "Demand firmly" .)