Demon in Mephistopheles personified, originally (3)
I believe the answer is:
imp
'demon' is the definition.
(I've seen this before)
'in mephistopheles personified originally' is the wordplay.
'originally' indicates taking the first letters.
The first letters of 'in mephistopheles personified' is 'IMP'.
(Other definitions for imp that I've seen before include "one likely to cause trouble" , "Little troublemaker" , "Sprite; add feathers to" , "Mischievous creature" , "Pixie" .)