Do not, at any time remove five from this! (4)
I believe the answer is:
neer
'this' is the definition.
Both the answer and definition are adverbs.
Maybe there's an association between them I don't understand?
'do not at any time remove five' is the wordplay.
'do not' becomes 'n' (I am not sure about this - if you are sure you should give a lot more credence to this answer).
'at any time' becomes 'ever' ('have you ever...' means 'have you at any time...').
'remove' is a deletion indicator.
'five' becomes 'V' (Roman numeral).
'ever' with 'v' taken away is 'eer'.
'n'+'eer'='NEER'
'from' acts as a link.
Can you help me to learn more?
(Other definitions for neer that I've seen before include "On no occasion" , "Of course not" , "At no time (poetic)" , "At no time (literary)" .)