Don’t include a single girl (4)
I believe the answer is:
miss
I believe this is a double definition.
'don't include' is the first definition.
(omit, or miss out)
'a single girl' is the second definition.
(I've seen this before)
(Other definitions for miss that I've seen before include "Don't hit" , "Girl or unmarried woman" , "One is as good as a mile" , "Pine for - young lady" , "Feel the absence of" .)