Duke providing barrier to limit queen’s dispute? (10)
I believe the answer is:
'dispute?' is the definition.
('difference' can be a synonym of 'dispute')
'duke providing barrier to limit queen's' is the wordplay.
'duke' becomes 'd'.
'providing' becomes 'if' (I've seen this in other clues).
'barrier' becomes 'fence' (fence is a kind of barrier**).
'to limit' is an insertion indicator.
'queen' becomes 'er' (abbreviation for Elizabeth Regina).
'fence' enclosing 'er' is 'ference'.
(Other definitions for difference that I've seen before include "Change" , "row" , "Diversity" , "Variation" , "Argument - dissimilarity" .)