E Sussex town, heading off Suffolk females? (4)
I believe the answer is:
ewes
'suffolk females?' is the definition.
(Suffolk is a breed of sheep)
'e sussex town heading off' is the wordplay.
'e sussex town' becomes 'lewes' (East Sussex town).
'heading off' means to remove the first letter.
'lewes' with its first letter taken off is 'EWES'.
(Other definitions for ewes that I've seen before include "Sheep (females)" , "members of flock" , "Adult sheep" , "Females in the flock" , "wool producers" .)