"Eye of ___ and toe of frog" (witches' ingredients in "Macbeth") (4)
I believe the answer is:
newt
(Other definitions for newt that I've seen before include "Amphibian - a Ken Livingstone pet?" , "Went to find kind of water lizard" , "Medical worker" , "Small tailed amphibian" , "Semiaquatic amphibian" .)