Fellow in Madrid taking top off upset ugly old ladies (5)
I believe the answer is:
senor
'fellow in madrid' is the definition.
(equivalent to 'mister' in Spanish)
I cannot understand how the remainder of the clue works.
(Other definitions for senor that I've seen before include "Spanish chap" , "address on the Costa del Sol" , "Snore like Spaniard" , "A Spanish mister" , "Spanish gent of Norse origin" .)