Followed calling with virtue (6)
I believe the answer is:
chased
'followed' is the definition.
(to chase someone is to follow them)
'calling with virtue' is the wordplay.
'calling' indicates a 'sounds like' (homophone) clue (how it might sound called out).
'with virtue' becomes 'chaste' (with virtue or virginity).
'chaste' is a homophone of 'CHASED'.
(Other definitions for chased that I've seen before include "Pursued at speed" , "Hunted" , "Wooed" , "Pursued or engraved" , "followed" .)