Fresh prey spotted up in the mountains (8)
I believe the answer is:
pyrenees
'the mountains' is the definition.
(I've seen this before)
'fresh prey spotted up' is the wordplay.
'fresh' indicates an anagram (a fresh re-ordering of the letters).
'spotted' becomes 'seen' (spot can mean to see or notice).
'up' says the letters should be written in reverse (in down clue: letters go upwards).
'seen' backwards is 'nees'.
'prey' with letters rearranged gives 'pyre'.
'pyre'+'nees'='PYRENEES'
'in' is the link.
(Other definitions for pyrenees that I've seen before include "Chain of mountains" , "Andorra in these mountains" , "Mountains between France and Spain" , "Mountain range between France and Spain" , "European mountains" .)