Funny, tight-lipped vocalisation made by leader leaving tour. (6)
I believe the answer is:
humour
'funny tight-lipped vocalisation' is the definition.
The definition suggests a singular noun which matches the answer.
'by leader leaving tour' is the wordplay.
'by' becomes 'hum' (I am not sure about this - if you are sure you should give a lot more credence to this answer).
'leader leaving' means to remove the first letter.
'tour' with its first letter taken away is 'our'.
'hum'+'our'='HUMOUR'
'made' acts as a link.
Can you help me to learn more?
(Other definitions for humour that I've seen before include "Hilarity" , "The quality of being funny" , "Wit" , "Bodily fluid" , "Give way to someone's wishes - it's funny" .)