Gamble with joker and queen (5)
I believe the answer is:
wager
'gamble' is the definition.
(I know that wager is a type of gamble)
'joker and queen' is the wordplay.
'joker' becomes 'wag' (I've seen this in another clue).
'and' says to put letters next to each other.
'queen' becomes 'ER' (abbreviation for Elizabeth Regina).
'wag'+'er'='WAGER'
'with' acts as a link.
(Other definitions for wager that I've seen before include "stake required" , "Punt" , "act for "The Gamblers"?" , "Bet, gamble" , "A bet or stake" .)