Gender not upset by church officer (6)
I believe the answer is:
sexton
'church officer' is the definition.
(I know that sexton is a type of church officer)
'gender not upset' is the wordplay.
'gender' becomes 'sex' (synonyms).
'upset' says the letters should be written in reverse.
'not' written backwards gives 'ton'.
'sex'+'ton'='SEXTON'
'by' acts as a link.
(Other definitions for sexton that I've seen before include "Job" , "One is responsible for church building" , "Church worker" , "church assistant" , "They went and told the -, and / The - tolled the bell (Thomas Hood)" .)