Great loss of pride? Not I (9)
I believe the answer is:
perdition
'great loss' is the definition.
(perdition could historically mean a severe loss)
'of pride? not i' is the wordplay.
'of' indicates anagramming the letters (the answer comes from or 'of' the letters).
'pride'+'not'+'i'='pridenoti'
'pridenoti' with letters rearranged gives 'PERDITION'.
(Other definitions for perdition that I've seen before include "Irrevocable spiritual ruin" , "Eternal damnation" , "Everlasting punishment" .)