Groups tramp through my local area last of all (5)
I believe the answer is:
phyla
'groups' is the definition.
(phylum is a kind of group)
'tramp through my local area last of all' is the wordplay.
'last of all' says to take the final letters.
The last letters of 'tramp through my local area' are 'PHYLA'.
(Other definitions for phyla that I've seen before include "Divisions in the kingdom" , "Divisions in botany" , "Classes immediately below those" , "Parts of the kingdom" , "whole lot of groups?" .)