Hate macho guards in charge (6)
I believe the answer is:
malice
'hate' is the definition.
(I know that malice is a type of malevolence)
'macho guards in charge' is the wordplay.
'macho' becomes 'male' (macho means aggressively masculine).
'guards' is an insertion indicator (inserted letters are guarded).
'in charge' becomes 'ic' (abbreviation for 'in charge').
'male' going around 'ic' is 'MALICE'.
(Other definitions for malice that I've seen before include "Bitterness" , "Hatred" , "Malevolence, spite" , "Spite, venom" , "'Ill-will, spite (6)'" .)