In Elizabethan times, a gamble (3)
I believe the answer is:
bet
'a gamble' is the definition.
(bet is a kind of gamble)
'in elizabethan times' is the wordplay.
'in' indicates a hidden word.
'BET' is hidden in the letters of 'elizabethan times'.
(Other definitions for bet that I've seen before include "Wager, back" , "22 across will accept one" , "Wager, or the sum waged" , "'Stake, wager (3)'" , "Wager, speculate" .)