In operation over the brook (2,6)
I believe the answer is:
on stream
'in operation' is the definition.
(I've seen this before)
'over the brook' is the wordplay.
'over' becomes 'on' (I've seen this in other clues**).
'the brook' becomes 'stream' (I've seen this before).
'on'+'stream'='ON-STREAM'
(Other definitions for on stream that I've seen before include "In operation or existence" , "in production" , "A monster (anag.)" , "In operation; storeman (anag.)" .)