Is that funny? Silence! (3)
I believe the answer is:
gag
I believe this is a double definition.
'is that funny?' is the first definition.
(gag can mean joke)
'silence' is the second definition.
(gagging is a kind of silencing)
(Other definitions for gag that I've seen before include "Make quiet" , "Shut up" , "Stop someone telling" , "Prevent free expression" , "Silence the joke" .)