James Bond's colleagues not alpha males (5)
I believe the answer is:
gents
'males' is the definition.
(gent is a kind of male)
'james bond's colleagues not alpha' is the wordplay.
'james bond's colleagues' becomes 'agents'.
'not' indicates named letters should be taken away.
'alpha' becomes 'a' (phonetic alphabet: alpha, bravo, charlie etc.).
'agents' with 'a' taken away is 'GENTS'.
(Other definitions for gents that I've seen before include "Men (colloq.)" , "Male loos" , "toilet" , "Public convenience" .)