Jot letter from Athens (4)
I believe the answer is:
iota
This clue is a double definition.
'jot' is the first definition.
(thesaurus)
'letter from athens' is the second definition.
(Greek letter)
(Other definitions for iota that I've seen before include "Tiny bit" , "Ninth Greek character" , "Grain" , "Gr. letter; small amount" , "Ninth letter of Greek alphabet, small amount" .)