Killer's aim with direct shot (9)
I believe the answer is:
matricide
'killer's' is the definition.
(someone who kills their mother)
'aim with direct shot' is the wordplay.
'with' is a charade indicator (letters next to each other).
'shot' indicates an anagram.
'aim' after 'direct' is 'directaim'.
'directaim' is an anagram of 'MATRICIDE'.
(Other definitions for matricide that I've seen before include "Diametric killing of a mother" , "Murderer" , "Killer" , "The killing of one's mother" , "Murder of one's mother" .)