Lay hose up operating theatre (9)
I believe the answer is:
playhouse
'theatre' is the definition.
(I know that theatre can be written as playhouse)
'lay hose up operating' is the wordplay.
'operating' indicates anagramming the letters.
'lay'+'hose'+'up'='layhoseup'
'layhoseup' anagrammed gives 'PLAYHOUSE'.
(Other definitions for playhouse that I've seen before include "Stage" , "army theatre" .)