Little Margaret is after fruit and spice (6)
I believe the answer is:
nutmeg
'spice' is the definition.
(I know that Nutmeg is a spice)
'little margaret is after fruit' is the wordplay.
'little margaret' becomes 'Meg' (short name for Margaret).
'is after' is a charade indicator (letters next to each other).
'fruit' becomes 'nut' (nut is a kind of fruit).
'meg' put after 'nut' is 'NUTMEG'.
'and' acts as a link.
(Other definitions for nutmeg that I've seen before include "Fragrant seed of tropical tree grated as spice" , "Hard seed of tropical tree grated for spice" , "Aromatic seed - tropical tree" , "Aromatic seed for grating" , "One grated as spice" .)