Little woman in scarlet performed again (5)
I believe the answer is:
redid
'performed again' is the definition.
('did' can be a synonym for 'performed' so perhaps 'redid' is OK for 'performed again')
'little woman in scarlet' is the wordplay.
'little woman' becomes 'di' (short for Diane, Diana etc).
'in' means one lot of letters goes inside another.
'scarlet' becomes 'red' ('red' can be a synonym of 'scarlet').
'di' going within 'red' is 'REDID'.
(Other definitions for redid that I've seen before include "Freshly decorated" , "Carried out again" , "Redecorated (a room)" , "Again prepared" , "tried again" .)