Mix of cast iron sample (4)
I believe the answer is:
stir
'mix' is the definition.
(I've seen this in another clue)
'of cast iron sample' is the wordplay.
'of' indicates the answer is hidden within the clue.
'STIR' is hidden in the letters of 'cast iron sample'.
(Other definitions for stir that I've seen before include "Create revolutionary movement" , "Act as an agitator" , "beat" , "Budge" , "Detention centre" .)