Mysterious English lake (5)
I believe the answer is:
eerie
'mysterious' is the definition.
(I've seen this before)
'english lake' is the wordplay.
'english' becomes 'e' (abbreviation).
'lake' becomes 'erie' (Lake Erie is one of the Canadian/US 'Great Lakes').
'e'+'erie'='EERIE'
(Other definitions for eerie that I've seen before include "Spooky, macabre" , "spine-chilling" , "Spine-tingling" , "Strange, frightening" , "Ghostly" .)