Crossword Genius

Number of layers of tissue (3)

Ross

I believe the answer is:

ply

(Other definitions for ply that I've seen before include "Thickness of yarn and wood" , "Work steadily at, say trade" , "Use or wield, tool or weapon" , "Travel a route regularly" , "A layer to work at diligently." .)

I've seen this clue in The Mirror.
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