Number one is unique (4)
I believe the answer is:
lone
'unique' is the definition.
(thesaurus)
'number one' is the wordplay.
'number' becomes 'l' (L is an example**).
'l'+'one'='LONE'
'is' is the link.
(Other definitions for lone that I've seen before include "He likes to operate by himself" , "Companionless" , "Solitary, without companions" , "Unaccompanied" , "Solitary or single" .)