O Brother, Where Art ___ ? (2000 film with George Clooney) (4)

I believe the answer is:

thou

(Other definitions for thou that I've seen before include "You (obs.)" , "1,000 (abbr)" , "Old-fashioned pronoun" , "'Holier than - - '" , "Biblical 'you'" .)

I've seen this clue in The Guardian.

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