Origin of Bond villain's accent (6)
I believe the answer is:
brogue
'accent' is the definition.
(I've seen this before)
'origin of bond villain's' is the wordplay.
'origin of' suggests taking the first letters.
'villain' becomes 'rogue' (rogue is a kind of villain).
The initial letter of 'bond' is 'b'.
'b'+'rogue'='BROGUE'
(Other definitions for brogue that I've seen before include "Stout leather shoe" , "Worn" , "afoot?" , "Kind of shoe" , "Outdoor shoe; accent" .)